Coding

Part:BBa_K2144100:Design

Designed by: Oskar hman   Group: iGEM16_Stockholm   (2016-10-12)


Coding sequence for Nuclease with His6 and LPXTG-tag regulated by T7-promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The LT-sequence was inserted with Overhang PCR.


Source

Nuclease is found in S.Aureus. The LT-sequence is ordered from IDT.

References